BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6478

Title: The N-domain of the transcription factor AbrB   PubMed: 15939023

Authors: Coles, Murray; Truffault, Vincent; Lupas, Andrei

Citation: Coles, Murray; Djuranovic, Sergej; Soding, Johannes; Frickey, Tancred; Koretke, Kristin; Truffault, Vincent; Martin, Joerg; Lupas, Andrei. "AbrB-like Transcription Factors Assume a Swapped Hairpin Fold that Is Evolutionarily Related to Double-Psi beta Barrels"  Structure (Camb) 13, 919-928 (2005).

Assembly members:
Transition state regulatory protein abrB, polymer, 53 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology   Host organism: Esherichia coli

Entity Sequences (FASTA):
Transition state regulatory protein abrB: MFMKSTGIVRKVDELGRVVI PIELRRTLGIAEKDALEIYV DDEKIILKKYKPN

Data sets:
Data typeCount
1H chemical shifts406
13C chemical shifts222
15N chemical shifts55

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Transition state regulatory protein abrB, chain A1
2Transition state regulatory protein abrB, chain B1

Entities:

Entity 1, Transition state regulatory protein abrB, chain A 53 residues - Formula weight is not available

1   METPHEMETLYSSERTHRGLYILEVALARG
2   LYSVALASPGLULEUGLYARGVALVALILE
3   PROILEGLULEUARGARGTHRLEUGLYILE
4   ALAGLULYSASPALALEUGLUILETYRVAL
5   ASPASPGLULYSILEILELEULYSLYSTYR
6   LYSPROASN

Samples:

sample_1: Transition state regulatory protein abrB, [U-15N], 0.5 mM; KPO4 20.0 mM; KCl 50.0 mM; NaN3 0.02%

sample_2: Transition state regulatory protein abrB, [U-15N], 0.25 mM; Transition state regulatory protein abrB, [U-13C], 0.25 mM; KPO4 20.0 mM; KCl 50.0 mM; NaN3 0.02%

sample_cond_1: pH: 5.8; temperature: 305 K

Experiments:

NameSampleSample stateSample conditions

Software:

XWINNMR v3.5 - collection, processing

NMRview - data analysis

X-PLOR v2.9.7 - structure solution, refinement

NMR spectrometers:

  • Bruker DMX 900 MHz

Related Database Links:

BMRB 17650 4281
PDB
DBJ BAA05272 BAI83483 BAL15827 BAM48968 BAM56238
EMBL CAA31307 CAA43955 CAB11813 CBI41163 CCF03572
GB AAA22195 AAP07140 AAP24090 AAS38971 AAT29114
PRF 1715209A
REF NP_387918 NP_829939 NP_842604 NP_976363 WP_000843034
SP P08874