BMRB Entry 6622
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR6622
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Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for HEWL-SMe
Deposition date: 2005-05-10 Original release date: 2008-07-16
Authors: Schlorb, Christian; Ackermann, Katrin; Richter, Christian; Wirmer, Julia; Schwalbe, Harald
Citation: Schlorb, Christian; Ackermann, Katrin; Richter, Christian; Wirmer, Julia; Schwalbe, Harald. "Heterologous expression of hen egg white lysozyme and resonance assignment of tryptophan side chains in its non-native states" J. Biomol. NMR 33, 95-104 (2005).
Assembly members:
Hen egg white lysozyme, polymer, 130 residues, 14444.3 Da.
Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: recombinant technology
Entity Sequences (FASTA):
Hen egg white lysozyme: MKVFGRXELAAAMKRHGLDN
YRGYSLGNWVXAAKFESNFN
TQATNRNTDGSTDYGILQIN
SRWWXNDGRTPGSRNLXNIP
XSALLSSDITASVNXAKKIV
SDGNGMNAWVAWRNRXKGTD
VQAWIRGXRL
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 402 |
| 15N chemical shifts | 116 |
| 13C chemical shifts | 332 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | hewl-sme | 1 |
Entities:
Entity 1, hewl-sme 130 residues - 14444.3 Da.
| 1 | MET | LYS | VAL | PHE | GLY | ARG | SMC | GLU | LEU | ALA | |
| 2 | ALA | ALA | MET | LYS | ARG | HIS | GLY | LEU | ASP | ASN | |
| 3 | TYR | ARG | GLY | TYR | SER | LEU | GLY | ASN | TRP | VAL | |
| 4 | SMC | ALA | ALA | LYS | PHE | GLU | SER | ASN | PHE | ASN | |
| 5 | THR | GLN | ALA | THR | ASN | ARG | ASN | THR | ASP | GLY | |
| 6 | SER | THR | ASP | TYR | GLY | ILE | LEU | GLN | ILE | ASN | |
| 7 | SER | ARG | TRP | TRP | SMC | ASN | ASP | GLY | ARG | THR | |
| 8 | PRO | GLY | SER | ARG | ASN | LEU | SMC | ASN | ILE | PRO | |
| 9 | SMC | SER | ALA | LEU | LEU | SER | SER | ASP | ILE | THR | |
| 10 | ALA | SER | VAL | ASN | SMC | ALA | LYS | LYS | ILE | VAL | |
| 11 | SER | ASP | GLY | ASN | GLY | MET | ASN | ALA | TRP | VAL | |
| 12 | ALA | TRP | ARG | ASN | ARG | SMC | LYS | GLY | THR | ASP | |
| 13 | VAL | GLN | ALA | TRP | ILE | ARG | GLY | SMC | ARG | LEU |
Samples:
sample_1: Hen egg white lysozyme, [U-13C; U-15N], 0.5 mM
condition_1: pH: 2.0; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_1 | not available | condition_1 |
Software:
No software information available
NMR spectrometers:
- BRUKER DRX 700 MHz
- BRUKER DRX 800 MHz
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