BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6718

Title: NMR structure of the human dematin headpiece S74E mutant   PubMed: 16472756

Authors: Jiang, Z.; Mcknight, C.

Citation: Jiang, Z.; Mcknight, C.. "A phosphorylation-induced conformation change in dematin headpiece"  Structure 14, 379-87 (2005).

Assembly members:
DHPs74e, polymer, 68 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
DHPs74e: PGLQIYPYEMLVVTNKGRTK LPPGVDRMRLERHLSAEDFS RVFAMSPEEFGKLALWKRNE LKKKAELF

Data sets:
Data typeCount
13C chemical shifts290
15N chemical shifts70
1H chemical shifts521

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1DHPs74e protein1

Entities:

Entity 1, DHPs74e protein 68 residues - Formula weight is not available

1   PROGLYLEUGLNILETYRPROTYRGLUMET
2   LEUVALVALTHRASNLYSGLYARGTHRLYS
3   LEUPROPROGLYVALASPARGMETARGLEU
4   GLUARGHISLEUSERALAGLUASPPHESER
5   ARGVALPHEALAMETSERPROGLUGLUPHE
6   GLYLYSLEUALALEUTRPLYSARGASNGLU
7   LEULYSLYSLYSALAGLULEUPHE

Samples:

sample_1: DHPs74e, [U-13C; U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_2: DHPs74e, [U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_3: DHPs74e, [U-10% 13C; U-15N], 1 mM; PHOSPHATE 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 10 mM; pH: 6.0; pressure: 1 atm; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
3D 13C-separated NOESYnot availablenot availablenot available
HNCA-Jnot availablenot availablenot available
HNCOCAnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available

Software:

Molmol v2K.2 - structure solution

CNS v1.0 - refinement

NMRPipe v2004 version - processing

xwinnmr v3.1 - collection

NMRView v5.0.4. - data analysis

NMR spectrometers:

  • BRUKER DMX 500 MHz

Related Database Links:

BMRB 5968
PDB
DBJ BAD92955 BAE28799 BAE34638 BAE37092 BAE38123
EMBL CAH92510 CAH93265 CAH93400
GB AAC50223 AAD34233 AAD38412 AAH06318 AAH16897
REF NP_001107607 NP_001107608 NP_001107609 NP_001107610 NP_001107611
SP Q08495 Q5R4B6 Q9WV69