BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6726

Title: Solution structure of self-sacrificing resistance protein CalC from Micromonospora echinospora

Authors: Singh, Shanteri; Thorson, Jon; Hager, Martin; Hallenga, Klaas; Markley, John

Citation: Singh, Shanteri; Hager, Martin; Zhang, Changsheng; Griffith, Bryon; Lee, Min; Hallenga, Klaas; Markley, John; Thorson, Jon. "Structural Insight into the Self-Sacrifice Mechanism of Enediyne Resistance"  Chem. Biol. 1, 451-460 (2006).

Assembly members:
CalC, polymer, 155 residues, Formula weight is not available

Natural source:   Common Name: Micromonospora echinospora   Taxonomy ID: 1877   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Micromonospora echinospora

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
CalC: NYDPFVRHSVTVKADRKTAF KTFLEGFPEWWPNNFRTTKV GAPLGVDKKGGRWYEIDEQG EEHTFGLIRKVDEPDTLVIG WRLNGFGRIDPDNSSEFTVT FVADGQKKTRVDVEHTHFDR MGTKHAKRVRNGMDKGWPTI LQSFQDKIDEEGAKK

Data sets:
Data typeCount
13C chemical shifts596
15N chemical shifts156
1H chemical shifts937

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1self-sacrificing resistance protein1

Entities:

Entity 1, self-sacrificing resistance protein 155 residues - Formula weight is not available

1   ASNTYRASPPROPHEVALARGHISSERVAL
2   THRVALLYSALAASPARGLYSTHRALAPHE
3   LYSTHRPHELEUGLUGLYPHEPROGLUTRP
4   TRPPROASNASNPHEARGTHRTHRLYSVAL
5   GLYALAPROLEUGLYVALASPLYSLYSGLY
6   GLYARGTRPTYRGLUILEASPGLUGLNGLY
7   GLUGLUHISTHRPHEGLYLEUILEARGLYS
8   VALASPGLUPROASPTHRLEUVALILEGLY
9   TRPARGLEUASNGLYPHEGLYARGILEASP
10   PROASPASNSERSERGLUPHETHRVALTHR
11   PHEVALALAASPGLYGLNLYSLYSTHRARG
12   VALASPVALGLUHISTHRHISPHEASPARG
13   METGLYTHRLYSHISALALYSARGVALARG
14   ASNGLYMETASPLYSGLYTRPPROTHRILE
15   LEUGLNSERPHEGLNASPLYSILEASPGLU
16   GLUGLYALALYSLYS

Samples:

sample_1: CalC, [U-95% 13C; U-90% 15N], 0.8 ± 0.1 mM; NaPO4 10 mM; NaCl 150 mM

conditions_1: ionic strength: 0.150 M; pH: 7.3; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
H(CCO)NHnot availablenot availablenot available
C(CO)NHnot availablenot availablenot available
H(C)CH TOCSYnot availablenot availablenot available
(H)CCH TOCSYnot availablenot availablenot available
NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian UNITY-INOVA 600 MHz
  • Varian UNITY-INOVA 800 MHz
  • Varian UNITY-INOVA 900 MHz

Related Database Links:

PDB
GB AAM70338