BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6731

Title: Backbone 1H, 13C, and 15N Chemical Shift Assignments for BOFC   PubMed: 16049010

Authors: Patterson, Hayley; Brannigan, James; Cutting, Simon; Wilson, Keith; Wilkinson, Anthony; Ab, Eiso; Diercks, Tammo; Folkers, Gert; de Jong, Rob; Truffault, Vincent

Citation: Patterson, Hayley; Brannigan, James; Cutting, Simon; Wilson, Keith; Wilkinson, Anthony; Ab, Eiso; Diercks, Tammo; Folkers, Gert; de Jong, Rob; Truffault, Vincent; Kaptein, Rob. "The structure of BofC, an inter-compartmental signalling factor in sporulation in Bacillus"  J. Biol. Chem. 280, 36214-36220 (2005).

Assembly members:
BofC, polymer, 140 residues, Formula weight is not available

Natural source:   Common Name: Bacillus subtilis   Taxonomy ID: 1423   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Bacillus subtilis

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
BofC: AEVEHYEPLQVHVQLEKVYL DGDVSIEHKHEKVFSMDDFW AAYAGWTLVEQKKGYVLFRK QMDDISPLSKVNGYIGVSDN GVISTFHGRPEPASEPIQSF FQIDLERLESHMQKNLLKGI PFRTKAEFEDVIEHMKTYSG

Data sets:
Data typeCount
13C chemical shifts617
15N chemical shifts140
1H chemical shifts958

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1BofC1

Entities:

Entity 1, BofC 140 residues - Formula weight is not available

1   ALAGLUVALGLUHISTYRGLUPROLEUGLN
2   VALHISVALGLNLEUGLULYSVALTYRLEU
3   ASPGLYASPVALSERILEGLUHISLYSHIS
4   GLULYSVALPHESERMETASPASPPHETRP
5   ALAALATYRALAGLYTRPTHRLEUVALGLU
6   GLNLYSLYSGLYTYRVALLEUPHEARGLYS
7   GLNMETASPASPILESERPROLEUSERLYS
8   VALASNGLYTYRILEGLYVALSERASPASN
9   GLYVALILESERTHRPHEHISGLYARGPRO
10   GLUPROALASERGLUPROILEGLNSERPHE
11   PHEGLNILEASPLEUGLUARGLEUGLUSER
12   HISMETGLNLYSASNLEULEULYSGLYILE
13   PROPHEARGTHRLYSALAGLUPHEGLUASP
14   VALILEGLUHISMETLYSTHRTYRSERGLY

Samples:

sample_one: BofC, [U-15N; U-13C], 0.5 mM; H2O 90%; D2O 10%; NaPO4 20 mM; EDTA 1 mM

condition_one: pH: 6.0; pressure: 1013 hPa; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablecondition_one

Software:

No software information available

NMR spectrometers:

  • unknown unknown 0 MHz

Related Database Links:

PDB
DBJ BAI86261 BAM53209 BAM58851 GAK80383
EMBL CAA63621 CAB14735 CAB75329 CCU59263 CEI58004
GB ADM38725 ADV93564 AEP87620 AEP91767 AFI29291
REF NP_390653 WP_003222663 WP_003229715 WP_003237539 WP_010331022
SP O05391