BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6740

Title: NMR solution structure of the Crisp domain of Tpx-1   PubMed: 16339766

Authors: Gibbs, G.; Scanlon, M.; Swarbrick, J.; Curtis, S.; Dulhunty, A.; O'Bryan, M.

Citation: Gibbs, G.; Scanlon, M.; Swarbrick, J.; Curtis, S.; Gallant, E.; Dulhunty, A.; O'Bryan, M.. "The cysteine-rich secretory protein domain of Tpx-1 is related to ion channel toxins and regulates ryanodine receptor Ca2+ signalling"  J. Biol. Chem. 281, 4156-4163 (2006).

Assembly members:
Cysteine-rich secretory protein-2, polymer, 57 residues, Formula weight is not available

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Cysteine-rich secretory protein-2: GSCASCPNNCENGLCTNSCD FEDLLSNCESLKTSAGCKHE LLKTKCQATCLCEDKIH

Data sets:
Data typeCount
13C chemical shifts78
15N chemical shifts58
1H chemical shifts293

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Cysteine-rich secretory protein-21

Entities:

Entity 1, Cysteine-rich secretory protein-2 57 residues - Formula weight is not available

1   GLYSERCYSALASERCYSPROASNASNCYS
2   GLUASNGLYLEUCYSTHRASNSERCYSASP
3   PHEGLUASPLEULEUSERASNCYSGLUSER
4   LEULYSTHRSERALAGLYCYSLYSHISGLU
5   LEULEULYSTHRLYSCYSGLNALATHRCYS
6   LEUCYSGLUASPLYSILEHIS

Samples:

sample_1: Cysteine-rich secretory protein-2, [U-13C; U-15], 1 mM; H2O 90%; D2O 10%

sample_2: Cysteine-rich secretory protein-2 1 mM; H2O 90%; D2O 10%

sample_3: Cysteine-rich secretory protein-2, [U-13C; U-15], 1 mM; D2O 100%

sample_cond_1: ionic strength: 1 mM; pH: 5.8; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D_15N-separated_NOESYnot availablenot availablenot available
3D_15N-separated TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
2D TOCSYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

XWINNMR v1, Bruker - collection, processing

SPARKY v3.101, Goddard Keller - data analysis

CYANA v1.06, Guntert - refinement

XPLOR-NIH v2.9.9, Clore Schwieters Kuszewski Tjandra - refinement

NMR spectrometers:

  • Bruker DRX 500 MHz

Related Database Links:

PDB
GenBank AAA40472 AAH49615 EDL23385
REF NP_033446
SWISS-PROT P16563