BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6742

Title: De-ubiquitinating function of ataxin-3: insights from the solution structure of the Josephin domain   PubMed: 16118278

Authors: Mao, Yuxin; Hodsdon, Michael; De Camilli, Pietro

Citation: Mao, Yuxin; Senic-Matuglia, Francesca; Di Fiore, Pier; Polo, Simona; Hodsdon, Michael; De Camilli, Pietro. "Deubiquitinating function of ataxin-3: Insights from the solution structure of the Josephin domain."  Proc. Natl. Acad. Sci. U. S. A. 102, 12700-12705 (2005).

Assembly members:
ataxin-3 josephin domain, polymer, 190 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
ataxin-3 josephin domain: MPLGSMESIFHEKQEGSLCA QHCLNNLLQGEYFSPVELSS IAHQLDEEERMRMAEGGVTS EDYRTFLQQPSGNMDDSGFF SIQVISNALKVWGLELILFN SPEYQRLRIDPINERSFICN YKEHWFTVRKLGKQWFNLNS LLTGPELISDTYLALFLAQL QQEGYSIFVVKGDLPDCEAD QLLQMIRVQQ

Data sets:
Data typeCount
13C chemical shifts750
15N chemical shifts199
1H chemical shifts1283

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ataxin-3 josephin domain1

Entities:

Entity 1, ataxin-3 josephin domain 190 residues - Formula weight is not available

1   METPROLEUGLYSERMETGLUSERILEPHE
2   HISGLULYSGLNGLUGLYSERLEUCYSALA
3   GLNHISCYSLEUASNASNLEULEUGLNGLY
4   GLUTYRPHESERPROVALGLULEUSERSER
5   ILEALAHISGLNLEUASPGLUGLUGLUARG
6   METARGMETALAGLUGLYGLYVALTHRSER
7   GLUASPTYRARGTHRPHELEUGLNGLNPRO
8   SERGLYASNMETASPASPSERGLYPHEPHE
9   SERILEGLNVALILESERASNALALEULYS
10   VALTRPGLYLEUGLULEUILELEUPHEASN
11   SERPROGLUTYRGLNARGLEUARGILEASP
12   PROILEASNGLUARGSERPHEILECYSASN
13   TYRLYSGLUHISTRPPHETHRVALARGLYS
14   LEUGLYLYSGLNTRPPHEASNLEUASNSER
15   LEULEUTHRGLYPROGLULEUILESERASP
16   THRTYRLEUALALEUPHELEUALAGLNLEU
17   GLNGLNGLUGLYTYRSERILEPHEVALVAL
18   LYSGLYASPLEUPROASPCYSGLUALAASP
19   GLNLEULEUGLNMETILEARGVALGLNGLN

Samples:

sample_1: ataxin-3 josephin domain, [U-100% 13C; U-100% 15N], 1.5 mM; potassium phospate buffer 20 mM

conditions_1: pH: 6.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D-HNCOsample_1not availableconditions_1
HNCAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HCACOnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
(HCA)CO(CA)NHnot availablenot availablenot available
CC(CO)NHnot availablenot availablenot available
HCC(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

BMRB 17333 6241
PDB
DBJ BAB18798 BAB25825 BAB55645 BAB55646 BAC27155
EMBL CAA72986
GB AAB33571 AAB63352 AAB63353 AAB63354 AAH33711
REF NP_001092686 NP_001116553 NP_001124408 NP_001158250 NP_001161386
SP O35815 P54252 Q9CVD2
TPG DAA17354