BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6749

Title: Solution structure of the NRSF/REST-mSin3B PAH1 complex

Authors: Nishimura, Yoshifumi; Nomura, Mitsuru

Citation: Nomura, Mitsuru; Uda-Tochio, Hiroko; Murai, Kiyohito; Mori, Nozomu; Nishimura, Yoshifumi. "The Neural Repressor NRSF/REST Binds the PAH1 Domain of the Sin3 Corepressor by Using its Distinct Short Hydrophobic Helix."  J. Mol. Biol. 354, 903-915 (2005).

Assembly members:
mSin3B PAH1, polymer, 80 residues, Formula weight is not available
NRSF/REST, polymer, 15 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
mSin3B PAH1: EKLPVHVEDALTYLDQVKIR FGSDPATYNGFLEIMKEFKS QSIDTPGVIRRVSQLFHEHP DLIVGFNAFLPLGYRIDIPK
NRSF/REST: APQLIMLANVALTGE

Data sets:
Data typeCount
13C chemical shifts364
15N chemical shifts78
1H chemical shifts642

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mSin3B PAH11
2NRSF/REST2

Entities:

Entity 1, mSin3B PAH1 80 residues - Formula weight is not available

1   GLULYSLEUPROVALHISVALGLUASPALA
2   LEUTHRTYRLEUASPGLNVALLYSILEARG
3   PHEGLYSERASPPROALATHRTYRASNGLY
4   PHELEUGLUILEMETLYSGLUPHELYSSER
5   GLNSERILEASPTHRPROGLYVALILEARG
6   ARGVALSERGLNLEUPHEHISGLUHISPRO
7   ASPLEUILEVALGLYPHEASNALAPHELEU
8   PROLEUGLYTYRARGILEASPILEPROLYS

Entity 2, NRSF/REST 15 residues - Formula weight is not available

1   ALAPROGLNLEUILEMETLEUALAASNVAL
2   ALALEUTHRGLYGLU

Samples:

sample_1: mSin3B PAH1, [U-13C; U-15N], 0.8 mM; NRSF/REST 0.8 mM; potassium phosphate buffer 20 mM

condition_1: pH: 7.5; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HBHA(CO)NHnot availablenot availablenot available
HN(CA)HAnot availablenot availablenot available
1H-13C HSQCnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
CCH-TOCSYnot availablenot availablenot available
13C/15N-FILTERED-NOESYnot availablenot availablenot available
13C/15N-FILTERED-TOCSYnot availablenot availablenot available

Software:

NMRPipe v2004 -

NMRView v5.0.4 -

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
DBJ BAB82460 BAD92987 BAE33741 BAG10902
GB AAA98503 AAB17211 AAB94893 AAB94894 AAC50114
PRF 2106430A
REF NP_001095350 NP_001180437 NP_005603 NP_035393 NP_113976
SP O54963 Q13127 Q8VIG1
TPG DAA28644 DAA28661