BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6752

Title: Backbone and sidechain 1H, 13C, and 15N Chemical Shift Assignments for the PH-PDZ tandem of alpha syntrophin   PubMed: 16252003

Authors: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie

Citation: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie. "Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin"  EMBO J. 24, 3985-3995 (2005).

Assembly members:
PH-PDZ tandem, polymer, 263 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
PH-PDZ tandem: ASGRRAPRTGLLELRCGAGS GAGGERWQRVLLSLAEDALT VSPADGEPGPEPEPAQLNGA AEPGAAPPQLPEALLLQRRR VTVRKADAGGLGISIKGGRE NKMPILISKIFKGLAADQTE ALFVGDAILSVNGEDLSSAT HDEAVQALKKTGKEVVLEVK YMKEVSPYFKNSAGGTSVGW DSPPASPLQRQPSSPGPQPR NLSEAKHVSLKMAYVSRRCT PTDPEPRYLEICAADGQDAV FLRAKDEASARSWAGAIQAQ IGT

Data sets:
Data typeCount
13C chemical shifts647
15N chemical shifts245
1H chemical shifts1650

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1PH-PDZ tandem1

Entities:

Entity 1, PH-PDZ tandem 263 residues - Formula weight is not available

1   ALASERGLYARGARGALAPROARGTHRGLY
2   LEULEUGLULEUARGCYSGLYALAGLYSER
3   GLYALAGLYGLYGLUARGTRPGLNARGVAL
4   LEULEUSERLEUALAGLUASPALALEUTHR
5   VALSERPROALAASPGLYGLUPROGLYPRO
6   GLUPROGLUPROALAGLNLEUASNGLYALA
7   ALAGLUPROGLYALAALAPROPROGLNLEU
8   PROGLUALALEULEULEUGLNARGARGARG
9   VALTHRVALARGLYSALAASPALAGLYGLY
10   LEUGLYILESERILELYSGLYGLYARGGLU
11   ASNLYSMETPROILELEUILESERLYSILE
12   PHELYSGLYLEUALAALAASPGLNTHRGLU
13   ALALEUPHEVALGLYASPALAILELEUSER
14   VALASNGLYGLUASPLEUSERSERALATHR
15   HISASPGLUALAVALGLNALALEULYSLYS
16   THRGLYLYSGLUVALVALLEUGLUVALLYS
17   TYRMETLYSGLUVALSERPROTYRPHELYS
18   ASNSERALAGLYGLYTHRSERVALGLYTRP
19   ASPSERPROPROALASERPROLEUGLNARG
20   GLNPROSERSERPROGLYPROGLNPROARG
21   ASNLEUSERGLUALALYSHISVALSERLEU
22   LYSMETALATYRVALSERARGARGCYSTHR
23   PROTHRASPPROGLUPROARGTYRLEUGLU
24   ILECYSALAALAASPGLYGLNASPALAVAL
25   PHELEUARGALALYSASPGLUALASERALA
26   ARGSERTRPALAGLYALAILEGLNALAGLN
27   ILEGLYTHR

Samples:

sample_1: PH-PDZ tandem, [U-13C; U-15N], 1 mM

sample_2: PH-PDZ tandem, [U-15N], 1 mM

conditions_1: pH: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15C-separated NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz

Related Database Links:

PDB
DBJ BAE23334
GB AAC52119 AAH18546 AAI69101 EDL06068 EDL06069
PRF 2208451B
REF NP_001094371 NP_033254 XP_006235388 XP_007640040
SP Q61234