BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6753

Title: Backbone 1H,13C, and 15N chemical shift assignments for the PH1 domain of alpha-syntrophin   PubMed: 16252003

Authors: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie

Citation: Yan, Jing; Wen, Wenyu; Xu, Weiguang; Long, Jia-fu; Adams, Marvin; Froehner, Stanley; Zhang, Mingjie. "Structure of the split PH domain and distinct lipid-binding properties of the PH-PDZ supramodule of alpha-syntrophin."  EMBO J. 24, 3985-3995 (2005).

Assembly members:
split PH domain, polymer, 178 residues, Formula weight is not available

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
split PH domain: ASGRRAPRTGLLELRCGAGS GAGGERWQRVLLSLAEDALT VSPADGEPGPEPEPAQLNGA AEPGAAPPQLPEALLLQREV SPYFKNSAGGTSVGWDSPPA SPLQRQPSSPGPQPRNLSEA KHVSLKMAYVSRRCTPTDPE PRYLEICAADGQDAVFLRAK DEASARSWAGAIQAQIGT

Data sets:
Data typeCount
13C chemical shifts483
15N chemical shifts162
1H chemical shifts1066

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1split PH domain1

Entities:

Entity 1, split PH domain 178 residues - Formula weight is not available

1   ALASERGLYARGARGALAPROARGTHRGLY
2   LEULEUGLULEUARGCYSGLYALAGLYSER
3   GLYALAGLYGLYGLUARGTRPGLNARGVAL
4   LEULEUSERLEUALAGLUASPALALEUTHR
5   VALSERPROALAASPGLYGLUPROGLYPRO
6   GLUPROGLUPROALAGLNLEUASNGLYALA
7   ALAGLUPROGLYALAALAPROPROGLNLEU
8   PROGLUALALEULEULEUGLNARGGLUVAL
9   SERPROTYRPHELYSASNSERALAGLYGLY
10   THRSERVALGLYTRPASPSERPROPROALA
11   SERPROLEUGLNARGGLNPROSERSERPRO
12   GLYPROGLNPROARGASNLEUSERGLUALA
13   LYSHISVALSERLEULYSMETALATYRVAL
14   SERARGARGCYSTHRPROTHRASPPROGLU
15   PROARGTYRLEUGLUILECYSALAALAASP
16   GLYGLNASPALAVALPHELEUARGALALYS
17   ASPGLUALASERALAARGSERTRPALAGLY
18   ALAILEGLNALAGLNILEGLYTHR

Samples:

sample_1: split PH domain, [U-13C; U-15N], 1 mM

sample_2: split PH domain, [U-15N], 1 mM

conditions_1: pH: 7; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HCCH-TOCSYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available
3D 15C-separated NOESYnot availablenot availablenot available

Software:

No software information available

NMR spectrometers:

  • Varian Inova 750 MHz

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