BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6757

Title: 1H and 13C Chemical Shift assignments for BVDV NS5A membrane anchor sequence [1-28]   PubMed: 16475810

Authors: Sapay, Nicolas; Montserret, Roland; Penin, Francois; Deleage, Gilbert

Citation: Sapay, Nicolas; Montserret, Roland; Chipot, Christophe; Brass, Volker; Moradpour, Darius; Deleage, Gilbert; Penin, Francois. "NMR Structure and Molecular Dynamics of the In-Plane Membrane Anchor of Nonstructural Protein 5A from Bovine Viral Diarrhea Virus"  Biochemistry 45, 2221-2233 (2006).

Assembly members:
BVDV NS5A[1-28], polymer, 28 residues, 3199.79 Da.

Natural source:   Common Name: Bovine Viral Diarrhea Virus   Taxonomy ID: 268305   Superkingdom: Viruses   Kingdom: Not applicable   Genus/species: Pestivirus Bovine viral diarrhea virus 1

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
BVDV NS5A[1-28]: SGNYVLDLIYSLHKQINRGL KKIVLGWA

Data typeCount
13C chemical shifts203
1H chemical shifts452

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1membrane anchor domain of BVDV NS5A1

Entities:

Entity 1, membrane anchor domain of BVDV NS5A 28 residues - 3199.79 Da.

1   SERGLYASNTYRVALLEUASPLEUILETYR
2   SERLEUHISLYSGLNILEASNARGGLYLEU
3   LYSLYSILEVALLEUGLYTRPALA

Samples:

TFE_50%: BVDV NS5A[1-28] 2 ± 0.1 mM; 2,2,2-TriFluoroEthanol-d2, TFE-d2 (>99%), 50 ± 1 % v/v; water 50 ± 1 % v/v; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.01 mM

SDS_100_mM: BVDV NS5A[1-28] 2 ± 0.1 mM; Sodium Dodecyl Sulfate (SDS)-d25, SDS-d25 (>98%), 100 ± 1 mM; water 100 ± 1 % v/v; 2,2-dimethyl-2-silapentane-5-sulfonic acid 0.01 mM

303K: pH: 4.5; temperature: 303 K

313K: pH: 4.5; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
TOCSY in TFE 50%not availablenot availablenot available
1H_13C_HSQC in TFE 50%not availablenot availablenot available
NOESY in TFE 50%not availablenot availablenot available
TOCSY in SDS 100 mMnot availablenot availablenot available
1H_13C_HSQC in SDS 100 mMnot availablenot availablenot available
NOESY in SDS 100 mMnot availablenot availablenot available

Software:

SPARKY v3.110, T. D. Goddard and D. G. Kneller - Chemical shift assignment

VNMR v6.1C, Varian - Spectra processing

NMR spectrometers:

  • Varian Unity-plus 500 MHz

Related Database Links:

PDB
GB AAC55984 AAG00378 AHG94955
SP Q96662