BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6760

Title: NMR assignment of the mTOR domain responsible for rapamycin binding   PubMed: 16456707

Authors: Veverka, Vaclav; Lennie, Gregor; Crabbe, Tom; Bird, Ian; Taylor, Richard; Carr, Mark

Citation: Veverka, Vaclav; Lennie, Gregor; Crabbe, Tom; Bird, Ian; Taylor, Richard; Carr, Mark. "NMR assignment of the mTOR domain responsible for rapamycin binding"  J. Biomol. NMR 36, 3-3 (2006).

Assembly members:
FRB domain, polymer, 126 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
FRB domain: MSYYHHHHHHDYDIPTTENL YFQGAMELIRVPILWHEMWH EGLEEASRLYFGERNVKGMF EVLEPLHAMMERGPQTLKET SFNQAYGRDLMEAQEWCRKY MKSGNVKDLTQAWDLYYHVF RRISKQ

Data sets:
Data typeCount
13C chemical shifts347
15N chemical shifts117
1H chemical shifts803

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1FRB domain1

Entities:

Entity 1, FRB domain 126 residues - Formula weight is not available

1   METSERTYRTYRHISHISHISHISHISHIS
2   ASPTYRASPILEPROTHRTHRGLUASNLEU
3   TYRPHEGLNGLYALAMETGLULEUILEARG
4   VALPROILELEUTRPHISGLUMETTRPHIS
5   GLUGLYLEUGLUGLUALASERARGLEUTYR
6   PHEGLYGLUARGASNVALLYSGLYMETPHE
7   GLUVALLEUGLUPROLEUHISALAMETMET
8   GLUARGGLYPROGLNTHRLEULYSGLUTHR
9   SERPHEASNGLNALATYRGLYARGASPLEU
10   METGLUALAGLNGLUTRPCYSARGLYSTYR
11   METLYSSERGLYASNVALLYSASPLEUTHR
12   GLNALATRPASPLEUTYRTYRHISVALPHE
13   ARGARGILESERLYSGLN

Samples:

sample_1: FRB domain, [U-15N], 0.1 mM

sample_2: FRB domain, [U-15N; U-13C], 0.1 mM

sample_3: FRB domain 0.1 mM

conditions_1: pH: 7.0; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
1H15N_HSQC_NOESYnot availablenot availableconditions_1
HNCACBnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HCCH-TOCSYnot availablenot availableconditions_1
1H13C_HSQC_NOESYnot availablenot availableconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 11471 16933
PDB
REF XP_007536126 XP_007895970