BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6761

Title: SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF AN N-TERMINAL UBIQUITIN-LIKE DOMAIN IN THE GLUT4-TETHERING PROTEIN, TUG   PubMed: 16501224

Authors: Tettamanzi, Cristina; Yu, Chenfei; Bogan, Jonathan; Hodsdon, Michael

Citation: Tettamanzi, Cristina; Yu, Chenfei; Bogan, Jonathan; Hodsdon, Michael. "SOLUTION STRUCTURE AND BACKBONE DYNAMICS OF AN N-TERMINAL UBIQUITIN-LIKE DOMAIN IN THE GLUT4-TETHERING PROTEIN, TUG"  Protein Sci. 15, 498-508 (2006).

Assembly members:
TUG-UBL1, polymer, 90 residues, Formula weight is not available

Natural source:   Common Name: house mouse   Taxonomy ID: not available   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
TUG-UBL1: MAAPAGGGGSAVSVLAPNGR RHTVKVTPSTVLLQVLEDTC RRQDFNPSEYDLKFQRTVLD LSLQWRFANLPNNAKLEMVP VSRSREGPEN

Data sets:
Data typeCount
13C chemical shifts378
15N chemical shifts83
1H chemical shifts605

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1UBL1 domain of TUG1

Entities:

Entity 1, UBL1 domain of TUG 90 residues - Formula weight is not available

1   METALAALAPROALAGLYGLYGLYGLYSER
2   ALAVALSERVALLEUALAPROASNGLYARG
3   ARGHISTHRVALLYSVALTHRPROSERTHR
4   VALLEULEUGLNVALLEUGLUASPTHRCYS
5   ARGARGGLNASPPHEASNPROSERGLUTYR
6   ASPLEULYSPHEGLNARGTHRVALLEUASP
7   LEUSERLEUGLNTRPARGPHEALAASNLEU
8   PROASNASNALALYSLEUGLUMETVALPRO
9   VALSERARGSERARGGLUGLYPROGLUASN

Samples:

sample_1: TUG-UBL1, [U-98% 13C; U-98% 15N], 0.75 mM; phosphate buffer 20 mM; NaCl 20 mM; sodium azide 0.05%; leupeptin 10 uM; pepstatin 10 uM; D20 5%

conditions_1: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableconditions_1
HN(CA)COsample_1not availableconditions_1
HNCACBsample_1not availableconditions_1
HN(CO)CAsample_1not availableconditions_1
HCACOsample_1not availableconditions_1
HCC(CO)NHsample_1not availableconditions_1
15N-TOCSYHSQCsample_1not availableconditions_1
HCCH-TOCSYsample_1not availableconditions_1
13C-NOESYHSQCsample_1not availableconditions_1
LRCCsample_1not availableconditions_1
15N-NOESY HSQCsample_1not availableconditions_1
(4D) 13C 15N HMQC-NOESY-HSQCsample_1not availableconditions_1

Software:

SPARKY v3.106 -

CYANA v1.0.5 -

NMRView v5.0.4 -

NMRPipe v1.0 -

NMR spectrometers:

  • Varian Inova 600 MHz

Related Database Links:

PDB
GB AAH19177 AAH22115 AAH31153 AAI62026 AAR01614
REF NP_081153 XP_001076577 XP_002724631 XP_003786587 XP_004404527
SP Q8VBT9