BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6766

Title: Complete assignment of a hypothetical protein pa0128 form Pseudomonas aeruginosa. Northeast Structural Genomics Consortium Target PaT1.   PubMed: 16703420

Authors: Srisailam, Sampath; Yee, Adelinda; Lukin, Jonathan; Lemak, Alexander; Bansal, Sonal; James, Prestegard; Arrowsmith, Cheryl

Citation: Srisailam, Sampath; Lukin, Jonathan; Lemak, Alexander; Yee, Adelinda; Arrowsmith, Cheryl. "Sequence Specific Resonance Assignment of a Hypothetical Protein PA0128 from Pseudomonas Aeruginosa"  J. Biomol. NMR 36, 27-27 (2006).

Assembly members:
phn-a like hypothetical protein, polymer, 138 residues, 12407 Da.
ZN, non-polymer, 65.409 Da.

Natural source:   Common Name: Bacteria   Taxonomy ID: 287   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Pseudomonas aeruginosa

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
phn-a like hypothetical protein: MGSSHHHHHHSSGRENLYFQ GHMVSTLPPCPQCNSEYTYE DGALLVCPECAHEWSPNEAA TASDDGKVIKDSVGNVLQDG DTITVIKDLKVKGSSLVVKV GTKVKNIRLVDGDHDIDCKI DGIGAMKLKSEFVRKVGS

Data sets:
Data typeCount
13C chemical shifts488
15N chemical shifts120
1H chemical shifts796

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1protein1
2Zn (II) ION2

Entities:

Entity 1, protein 138 residues - 12407 Da.

1   METGLYSERSERHISHISHISHISHISHIS
2   SERSERGLYARGGLUASNLEUTYRPHEGLN
3   GLYHISMETVALSERTHRLEUPROPROCYS
4   PROGLNCYSASNSERGLUTYRTHRTYRGLU
5   ASPGLYALALEULEUVALCYSPROGLUCYS
6   ALAHISGLUTRPSERPROASNGLUALAALA
7   THRALASERASPASPGLYLYSVALILELYS
8   ASPSERVALGLYASNVALLEUGLNASPGLY
9   ASPTHRILETHRVALILELYSASPLEULYS
10   VALLYSGLYSERSERLEUVALVALLYSVAL
11   GLYTHRLYSVALLYSASNILEARGLEUVAL
12   ASPGLYASPHISASPILEASPCYSLYSILE
13   ASPGLYILEGLYALAMETLYSLEULYSSER
14   GLUPHEVALARGLYSVALGLYSER

Entity 2, Zn (II) ION - Zn - 65.409 Da.

1   ZN

Samples:

sample_1: hypothetical protein pa0128, [U-15N; U-13C], 1 ± 0.2 mM; sodium phosphates 20 ± 0.2 mM; NaCl 400 ± 0.2 mM; DTT 15 ± 0.2 mM; Benzamidine 1 ± 0.2 mM; Sodium Azide 0.01 ± 0.01 %; Zinc Sulfate 20 ± 0.1 uM; D2O 5 ± 0.1 %

conditions_1: ionic strength: 0.4 M; pH: 6.5; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
HNCOsample_1not availableconditions_1
Aromatic 13C-Noesysample_1not availableconditions_1
CBCAcoNHsample_1not availableconditions_1
HNCAsample_1not availableconditions_1
HNCBsample_1not availableconditions_1
HNcoCAsample_1not availableconditions_1
HBHAcoNHsample_1not availableconditions_1
CCTOCSY_NHsample_1not availableconditions_1
HCCTOCSY_NHsample_1not availableconditions_1
13C HSQsample_1not availableconditions_1
HCCHCOSYsample_1not availableconditions_1
15N NOESYsample_1not availableconditions_1
13C NOESYsample_1not availableconditions_1
hcbcaconh PRO exptsample_1not availableconditions_1
hcangp3d PRO exptsample_1not availableconditions_1

Software:

SPARKY v3.95 -

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
DBJ BAK87024 BAP19254 BAP48132 GAA19818 GAJ54686
EMBL CAW24856 CCQ86833 CDH68429 CDH74517 CDI88258
GB AAG03518 ABJ15085 ABR86035 AEO72619 AFM62426
REF NP_248818 WP_003083779 WP_003101229 WP_014603467 WP_023081666