BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6771

Title: Solution structure of a "retroevolved" lambda Cro monomer reveals the basis for increased monomer stability and reduced dimerization   PubMed: 16700549

Authors: Newlove, T.; LeFevre, K.; Cordes, M.

Citation: Newlove, T.; Atkinson, K.; Van Dorn, L.; Cordes, M.. "A trade between similar but nonequivalent intrasubunit and intersubunit contacts in cro dimer evolution"  Biochemistry 45, 6379-6391 (2006).

Assembly members:
Regulatory protein cro, polymer, 66 residues, Formula weight is not available

Natural source:   Common Name: Enterobacteria phage lambda   Taxonomy ID: 10710   Superkingdom: Viruses   Kingdom: not available   Genus/species: Lambda-like viruses Bacteriophage lambda

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Regulatory protein cro: MEQRITLKDYAMRFGQTKTA KDLGVQQSAINKWIHAGRKI FLTINADGSVYAEEVKPDPS NKKTTA

Data sets:
Data typeCount
13C chemical shifts227
15N chemical shifts74
1H chemical shifts482

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Regulatory protein cro1

Entities:

Entity 1, Regulatory protein cro 66 residues - Formula weight is not available

1   METGLUGLNARGILETHRLEULYSASPTYR
2   ALAMETARGPHEGLYGLNTHRLYSTHRALA
3   LYSASPLEUGLYVALGLNGLNSERALAILE
4   ASNLYSTRPILEHISALAGLYARGLYSILE
5   PHELEUTHRILEASNALAASPGLYSERVAL
6   TYRALAGLUGLUVALLYSPROASPPROSER
7   ASNLYSLYSTHRTHRALA

Samples:

sample_1: Regulatory protein cro, [U-13C], 2.5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_2: Regulatory protein cro, [U-15N], 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_3: Regulatory protein cro 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; H2O 90%; D2O 10%

sample_4: Regulatory protein cro, [U-15N], 5 mM; Na-phosphate 50 mM; sodium azide 0.01%; TSP 1 mM; D2O 100%

sample_cond_1: pH: 5.3; pressure: 1 atm; temperature: 293 K

sample_cond_2: pH: 6.1; pressure: 1 atm; temperature: 293 K

sample_cond_3: pH: 6.1; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-separated NOESYnot availablenot availablenot available
HNHAnot availablenot availablenot available
HNHBnot availablenot availablenot available
HSQC (amide hydrogen exchange)not availablenot availablenot available
3D 13C-separated NOESYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available

Software:

xwinnmr v3.1 - collection

NMRPipe v1.8 - processing

NMRView v4.1.3 - data analysis

CNS v1.1 - refinement, structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

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