BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6776

Title: Assignment of 1H, 15N and 13C resonances of the bacterial protein YFHJ   PubMed: 16698547

Authors: Pastore, Chiara; Kelly, Geoff; Adinolfi, Salvatore; McCormick, John; Pastore, Annalisa

Citation: Pastore, Chiara; Adinolfi, Salvatore; Huynen, Martjin; Rybin, Vladimir; Martin, Stephen; Mayer, Mathias; Bukau, Bernds; Pastore, Annalisa. "YfhJ, a Molecular Adaptor in Iron-Sulfur Cluster Formation or a Frataxin-like Protein?"  Structure 14, 857-867 (2006).

Assembly members:
YFHJ, polymer, 66 residues, 7731.46 Da.

Natural source:   Common Name: Escherichia Coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
YFHJ: MGLKWTDSREIGEALYDAYP DLDPKTVRFTDMHQWICDLE DFDDDPQASNEKILEAILLV WLDEAE

Data sets:
Data typeCount
13C chemical shifts300
15N chemical shifts69
1H chemical shifts474

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1YFHJ1

Entities:

Entity 1, YFHJ 66 residues - 7731.46 Da.

1   METGLYLEULYSTRPTHRASPSERARGGLU
2   ILEGLYGLUALALEUTYRASPALATYRPRO
3   ASPLEUASPPROLYSTHRVALARGPHETHR
4   ASPMETHISGLNTRPILECYSASPLEUGLU
5   ASPPHEASPASPASPPROGLNALASERASN
6   GLULYSILELEUGLUALAILELEULEUVAL
7   TRPLEUASPGLUALAGLU

Samples:

sample_1: YFHJ, [U-98% 15N], 0.5 mM; phosphate 10 mM; NaCl 50%; H20 90%; D2O 10%

sample_2: YFHJ, [U-99% 13C; U-98 15N], 0.6 mM; phosphate 10 mM; NaCl 50 mM; H2O 90%; D2O 10%

conditions_1: pH: 6.5; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
1H_13C_NOESY-HSQCnot availablenot availableconditions_1
1H_15N_NOESY-HSQCnot availablenot availableconditions_1
1H15N_TOCSY-HSQCnot availablenot availableconditions_1
1H15N_HSQCnot availablenot availableconditions_1
1H13C_HSQCnot availablenot availableconditions_1
HCCH_TOCSYnot availablenot availableconditions_1
CBCANHnot availablenot availableconditions_1
CBCACONHnot availablenot availableconditions_1
HNCAnot availablenot availableconditions_1
HNCOCAnot availablenot availableconditions_1
HNCOnot availablenot availableconditions_1
HNHAnot availablenot availableconditions_1

Software:

XEASY - Assignment

NMRDraw - Data Processing

NMRPipe - Data Processing

NMR spectrometers:

  • Varian INOVA 600 MHz
  • Varian UNITY-plus 500 MHz
  • Varian INOVA 800 MHz

Related Database Links:

PDB
DBJ BAA16414 BAB36813 BAG78334 BAI26769 BAI31798
EMBL CAP76976 CAQ32897 CAQ88192 CAQ99415 CAR03966
GB AAC75577 AAG57638 AAN44070 AAN81499 AAP17895
REF NP_289081 NP_311417 NP_417019 NP_708363 NP_754931
SP P0C0L9 P0C0M0 P0C0M1 Q8FF46