BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6780

Title: NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae   PubMed: 16341755

Authors: Sari, Nese; Doseeva, Victoria; Subarian, Karen; Herzberg, Osnat; Orban, John

Citation: Sari, Nese; Yeh, Deok; Doseeva, Victoria; Surabian, Karen; Herzberg, Osnat; Orban, John. "NMR Assignment of HI1506, a novel two-domain protein from Haemophilus influenzae"  J. Biomol. NMR 33, 281-281 (2005).

Assembly members:
hi1506, polymer, 131 residues, Formula weight is not available

Natural source:   Common Name: Haemophilus influenza   Taxonomy ID: 727   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Haemophilus influenzae

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
hi1506: GSHMDKTFCVVVQNRIKEGY RRAGFSFHLGDNSLAAVSES QLAQLKADPRLVVQITETGS QEGGEGLSKEPAGSDEQKQL RADPPSTDLNTFTVEQLKAQ LTERGITFKQSATKAELIAL FAPADGEKSEA

Data sets:
Data typeCount
13C chemical shifts520
15N chemical shifts129
1H chemical shifts790

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1hi15061

Entities:

Entity 1, hi1506 131 residues - Formula weight is not available

1   GLYSERHISMETASPLYSTHRPHECYSVAL
2   VALVALGLNASNARGILELYSGLUGLYTYR
3   ARGARGALAGLYPHESERPHEHISLEUGLY
4   ASPASNSERLEUALAALAVALSERGLUSER
5   GLNLEUALAGLNLEULYSALAASPPROARG
6   LEUVALVALGLNILETHRGLUTHRGLYSER
7   GLNGLUGLYGLYGLUGLYLEUSERLYSGLU
8   PROALAGLYSERASPGLUGLNLYSGLNLEU
9   ARGALAASPPROPROSERTHRASPLEUASN
10   THRPHETHRVALGLUGLNLEULYSALAGLN
11   LEUTHRGLUARGGLYILETHRPHELYSGLN
12   SERALATHRLYSALAGLULEUILEALALEU
13   PHEALAPROALAASPGLYGLULYSSERGLU
14   ALA

Samples:

sample_1: hi1506, [U-13C; U-15N], 1.0 ± 0.1 mM; potassium phosphate 50 mM; NaCl 100 mM; DTT 1 mM; D2O 90%; H2O 10%

conditions_1: ionic strength: 100 mM; pH: 7; pressure: 760 mmHg; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
15n-hsqcsample_1not availableconditions_1
13c-hsqcsample_1not availableconditions_1
hbcbhdsample_1not availableconditions_1
hbcbhdhesample_1not availableconditions_1
hncacbsample_1not availableconditions_1
cbcaconhsample_1not availableconditions_1
hncosample_1not availableconditions_1
hbhaconhsample_1not availableconditions_1
hcconhsample_1not availableconditions_1
cconhsample_1not availableconditions_1
15n-noesy-hsqcsample_1not availableconditions_1
13c-noesy-hsqcsample_1not availableconditions_1

Software:

SPARKY v2.0 - manual peak assignments

NMR spectrometers:

  • Bruker AVANCE 600 MHz

Related Database Links:

PDB
GB AAC23165 EEW76901
REF WP_005693527
SP P44228