BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6894

Title: New insights into binding of the possible cancer target RalGDS

Authors: Gronwald, W.; Maurer, T.; Fuechsl, R.; Wohlgemuth, S.; Herrmann, C.; Kalbitzer, H.

Citation: Gronwald, W.; Maurer, T.; Fuechsl, R.; Wohlgemuth, S.; Herrmann, C.; Kalbitzer, H.. "New insights into binding of the possible cancer target RalGDS"  . ., .-..

Assembly members:
Ral guanine nucleotide dissociation stimulator, polymer, 87 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Ral guanine nucleotide dissociation stimulator: DCCIIRVSLDVDNGNMYKSI LVTSQDKAPAVIRKAMDKHN LEEEEPEDYELLQILSDDRK LKIPENANVFYAMNSTANYD FVLKKRT

Data sets:
Data typeCount
13C chemical shifts349
15N chemical shifts93
1H chemical shifts605

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Ral guanine nucleotide dissociation stimulator1

Entities:

Entity 1, Ral guanine nucleotide dissociation stimulator 87 residues - Formula weight is not available

1   ASPCYSCYSILEILEARGVALSERLEUASP
2   VALASPASNGLYASNMETTYRLYSSERILE
3   LEUVALTHRSERGLNASPLYSALAPROALA
4   VALILEARGLYSALAMETASPLYSHISASN
5   LEUGLUGLUGLUGLUPROGLUASPTYRGLU
6   LEULEUGLNILELEUSERASPASPARGLYS
7   LEULYSILEPROGLUASNALAASNVALPHE
8   TYRALAMETASNSERTHRALAASNTYRASP
9   PHEVALLEULYSLYSARGTHR

Samples:

sample_1: Ral guanine nucleotide dissociation stimulator 1.0 mM; potassium buffer 20 mM; dithioerythritol (DTE) 2 mM; EDTA 0.5 mM; NaN3 0.5 mM; 2,2-dimethyl-2-silapentane sulfonic acid(DSS) 0.1 mM; H2O 95%; D2O 5%

sample_2: Ral guanine nucleotide dissociation stimulator, [U-13C; U-15N], 2.9 mM; potassium buffer 20 mM; dithioerythritol (DTE) 2 mM; EDTA 0.5 mM; NaN3 0.5 mM; 2,2-dimethyl-2-silapentane sulfonic acid(DSS) 0.1 mM; H2O 95%; D2O 5%

sample_3: Ral guanine nucleotide dissociation stimulator 1.0 mM; potassium buffer 20 mM; dithioerythritol (DTE) 2 mM; EDTA 0.5 mM; NaN3 0.5 mM; 2,2-dimethyl-2-silapentane sulfonic acid(DSS) 0.1 mM; H2O 95%; D2O 100%

sample_cond_1: ionic strength: 20 mM; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D NOESYnot availablenot availablenot available
3D 15N-separated NOESYnot availablenot availablenot available

Software:

xwinnmr v2.6 - collection, processing

AUREMOL v2.2 - data analysis, iterative matrix relaxation

CNS v1.1 - structure solution

X-PLOR NIH v2.9.6 - refinement

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links: