BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 6898

Title: NMR structure of the peptide linked to the genome (VPg) of poliovirus   PubMed: 16540201

Deposition date: 2005-11-10 Original release date: 2007-02-06

Authors: Schein, C.; Oezguen, N.

Citation: Schein, C.; Oezguen, N.; Volk, D.; Garimella, R.; Paul, A.; Braun, W.. "NMR structure of the viral peptide linked to the genome (VPg) of poliovirus"  Peptides 27, 1676-1684 (2006).

Assembly members:
Genome linked protein VPg, polymer, 22 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Genome linked protein VPg: GAYTGLPNKKPNVPTIRTAK VQ

Data sets:
Data typeCount
1H chemical shifts276

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Genome linked protein VPg1

Entities:

Entity 1, Genome linked protein VPg 22 residues - Formula weight is not available

1   GLYALATYRTHRGLYLEUPROASNLYSLYS
2   PROASNVALPROTHRILEARGTHRALALYS
3   VALGLN

Samples:

sample_1: Genome linked protein VPg 2.78 mM; sodium phosphate buffer 0.01 M; deuterated Trimethylamine oxide 1 M; D2O 10%; H2O 90%

sample_2: Genome linked protein VPg 3.7 mM; sodium phosphate buffer 0.01 M; D2O 10%; H2O 90%

sample_cond_1: ionic strength: 10 mM; pH: 7.2; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D TOCSYnot availablenot availablenot available
2D NOESYnot availablenot availablenot available
DQF-COSYnot availablenot availablenot available

Software:

NOAH v2.0 - structure solution

DIAMOD v2.2 - refinement, structure solution

FELIX - data analysis

NMR spectrometers:

  • Varian UNITYplus 600 MHz

Related Database Links:

PDB
DBJ BAD72853 BAD72854 BAD72855 BAD72856 BAE20395
EMBL CAA24445 CAA24446 CAA24456 CAA24457 CAA24461
GB AAA46911 AAG27151 AAG27168 AAN85442 AAP37263
PRF 0707268A 0908283A 0908283B
REF NP_041277 NP_740475 YP_007353733
SP P03300 P03301