BMRB Entry 7049
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR7049
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Title: 1H chemical shifts of the fifth transmembrane segment of Na,K-ATPase in SDS micelles PubMed: 16904671
Deposition date: 2006-03-31 Original release date: 2006-08-31
Authors: Underhaug, Jarl; Jakobsen, Louise; Fojan, Peter; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.
Citation: Underhaug, Jarl; Jakobsen, Louise; Esmann, Mikael; Malmendal, Anders; Nielsen, Niels Chr.. "NMR studies of the fifth transmembrane segment of Na(+),K(+)-ATPase reveals a non-helical ion-binding region." FEBS Lett. 580, 4777-4783 (2006).
Assembly members:
M5 peptide from Na,K-ATPase, polymer, 28 residues, Formula weight is not available
Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo Sapiens
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
M5 peptide from Na,K-ATPase: KKSIAYTLTSNIPEITPFLI
FIIANIPL
- assigned_chemical_shifts
| Data type | Count |
| 15N chemical shifts | 4 |
| 1H chemical shifts | 209 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | M5 | 1 |
Entities:
Entity 1, M5 28 residues - Formula weight is not available
| 1 | LYS | LYS | SER | ILE | ALA | TYR | THR | LEU | THR | SER | ||||
| 2 | ASN | ILE | PRO | GLU | ILE | THR | PRO | PHE | LEU | ILE | ||||
| 3 | PHE | ILE | ILE | ALA | ASN | ILE | PRO | LEU |
Samples:
2.5mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 2.5 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM
5.0mM: M5 peptide from Na,K-ATPase, [15N]-ALA5,LEU8,LEU19,ALA24, 5.0 mM; SDS-d25 400 mM; NaCl 100 mM; Sodium phosphate 20 mM
conditions_1: pH: 7.0; temperature: 313 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| DQF-COSY | 5.0mM | not available | conditions_1 |
| TOCSY | 2.5mM | not available | conditions_1 |
| NOESY | 2.5mM | not available | conditions_1 |
| HSQC-NOESY | 2.5mM | not available | conditions_1 |
Software:
No software information available
NMR spectrometers:
- Bruker Avance 400.13 MHz
- Varian Inova 799.81 MHz
Related Database Links:
| PDB | |
| DBJ | BAA00061 BAE28712 BAE29635 BAE34957 BAG10675 |
| EMBL | CAA26581 CAA26582 CAA27576 CAA27840 CAA29306 |
| GB | AAA19022 AAA31002 AAA40775 AAA41671 AAA48607 |
| PRF | 1109241A 1208322A 1210234A 1309271A 1513185A |
| REF | NP_000692 NP_001003306 NP_001009360 NP_001070266 NP_001082580 |
| SP | P04074 P05023 P05024 P06685 P09572 |
| TPG | DAA31562 |
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