BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 7419

Title: SOLUTION STRUCTURE OF AMINO TERMINAL DOMAIN OF HUMAN DNA POLYMERASE EPSILON SUBUNIT B REVEALS ITS HOMOLOGY TO C-DOMAINS OF AAA PROTEINS.   PubMed: 18676977

Authors: Nuutinen, T.; Fredriksson, K.; Tossavainen, H.; Pospiech, H.; Pirila, P.; Permi, P.; Annila, A.; Syvaoja, J.

Citation: Nuutinen, Tarmo; Tossavainen, Helena; Fredriksson, Kai; Pirila, Paivi; Permi, Perttu; Pospiech, Helmut; Syvaoja, Juhani. "The solution structure of the amino-terminal domain of human DNA polymerase epsilon subunit B is homologous to C-domains of AAA+ proteins"  Nucleic Acids Res. 36, 5102-5110 (2008).

Assembly members:
AAA_protein, polymer, 99 residues, Formula weight is not available

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
AAA_protein: MGSSHHHHHHSQDPNSSSAR LQVDMAPERLRSRALSAFKL RGLLLRGEAIKYLTEALQSI SELELEDKLEKIINAVEKQP LSSNMIERSVVEAAVQESS

Data sets:
Data typeCount
13C chemical shifts386
15N chemical shifts90
1H chemical shifts646

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1AAA_protein1

Entities:

Entity 1, AAA_protein 99 residues - Formula weight is not available

1   METGLYSERSERHISHISHISHISHISHIS
2   SERGLNASPPROASNSERSERSERALAARG
3   LEUGLNVALASPMETALAPROGLUARGLEU
4   ARGSERARGALALEUSERALAPHELYSLEU
5   ARGGLYLEULEULEUARGGLYGLUALAILE
6   LYSTYRLEUTHRGLUALALEUGLNSERILE
7   SERGLULEUGLULEUGLUASPLYSLEUGLU
8   LYSILEILEASNALAVALGLULYSGLNPRO
9   LEUSERSERASNMETILEGLUARGSERVAL
10   VALGLUALAALAVALGLNGLUSERSER

Samples:

sample: entity_1 mM

sample_conditions_1: pH: 6.62; pressure: 1.0 atm; temperature: 298.0 K

Experiments:

NameSampleSample stateSample conditions
HNCAsampleisotropicsample_conditions_1
HNCACBsampleisotropicsample_conditions_1
HNCOCACBsampleisotropicsample_conditions_1
HNCOsampleisotropicsample_conditions_1
HCCONHsampleisotropicsample_conditions_1
CCCONHsampleisotropicsample_conditions_1
HCCH-COSYsampleisotropicsample_conditions_1
NOESY-HSQCsampleisotropicsample_conditions_1

Software:

CYANA - structure solution

AMBER v8 - structure solution

ProcheckNMR vNMR - structure solution

NMR spectrometers:

  • VARIAN UNITYINOVA 800 MHz

Related Database Links:

PDB
DBJ BAG56707 BAJ21084
GB AAC39610 AAC51920 AAI12963 AAI26219 AAI26221
REF NP_001184259 NP_001184260 NP_002683 XP_003267698 XP_003267699
SP P56282