BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 7435

Title: HVS ORF57 8-120 backbone assignment, in complex with REF2-I 54-155.   PubMed: 21253573

Authors: Tunnicliffe, Richard; Golovanov, Alexander; Wilson, Stuart; Hautbergue, Guillaume

Citation: Tunnicliffe, Richard; Hautbergue, Guillaume; Kalra, Priti; Jackson, Brian; Whitehouse, Adrian; Wilson, Stuart; Golovanov, Alexander. "Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57."  PLoS Pathog. 7, e1001244-. (2011).

Assembly members:
REF_54-155, polymer, 124 residues, 13596.237 Da.
ORF57_8-120, polymer, 135 residues, 4268.970 Da.

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
REF_54-155: MASMTGGQQMGRDPDKWQHD LFDSGCGGGEGVETGAKLLV SNLDFGVSDADIQELFAEFG TLKKAAVDYDRSGRSLGTAD VHFERRADALKAMKQYKGVP LDGRPMDIQLVASQIDLEHH HHHH
ORF57_8-120: MASMTGGQQMGRDPGISSDD DFDSSDSSSDEEESDTSPQI MKSDVTMASPPSTPEPSLDV SASTSNLKRERQRSPITWEH QSPLSRVYRSPSPMRFGKRP RISSNSTSRSCKTSWADRVR EAAAQRRLEHHHHHH

Data sets:
Data typeCount
15N chemical shifts104
1H chemical shifts104

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1REF_54-1551
2ORF57_8-1202

Entities:

Entity 1, REF_54-155 124 residues - 13596.237 Da.

Construct contains the following non-REF sequences: N-terminal T7 tag: MASMTGGQQMGRDP (assigned residue numbers 40-53) C-terminal His tag: LEHHHHHH (assigned residue numbers 156-163)

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGASPPROASPLYSTRPGLNHISASP
3   LEUPHEASPSERGLYCYSGLYGLYGLYGLU
4   GLYVALGLUTHRGLYALALYSLEULEUVAL
5   SERASNLEUASPPHEGLYVALSERASPALA
6   ASPILEGLNGLULEUPHEALAGLUPHEGLY
7   THRLEULYSLYSALAALAVALASPTYRASP
8   ARGSERGLYARGSERLEUGLYTHRALAASP
9   VALHISPHEGLUARGARGALAASPALALEU
10   LYSALAMETLYSGLNTYRLYSGLYVALPRO
11   LEUASPGLYARGPROMETASPILEGLNLEU
12   VALALASERGLNILEASPLEUGLUHISHIS
13   HISHISHISHIS

Entity 2, ORF57_8-120 135 residues - 4268.970 Da.

The construct contains the following non-ORF57 sequence at N-termini: MASMTGGQQMGRDP (assigned residue numbers -4 to 7) Plus the following at C-termini: LEHHHHHH (assigned residue numbers 121 to 128)

1   METALASERMETTHRGLYGLYGLNGLNMET
2   GLYARGASPPROGLYILESERSERASPASP
3   ASPPHEASPSERSERASPSERSERSERASP
4   GLUGLUGLUSERASPTHRSERPROGLNILE
5   METLYSSERASPVALTHRMETALASERPRO
6   PROSERTHRPROGLUPROSERLEUASPVAL
7   SERALASERTHRSERASNLEULYSARGGLU
8   ARGGLNARGSERPROILETHRTRPGLUHIS
9   GLNSERPROLEUSERARGVALTYRARGSER
10   PROSERPROMETARGPHEGLYLYSARGPRO
11   ARGILESERSERASNSERTHRSERARGSER
12   CYSLYSTHRSERTRPALAASPARGVALARG
13   GLUALAALAALAGLNARGARGLEUGLUHIS
14   HISHISHISHISHIS

Samples:

sample_1: ORF57 8-120, [U-99% 13C; U-99% 15N], 1 ± 0.05 mM; REF 54-155 2 ± 0.05 mM; phosphate buffer 20 mM; NaCl 50 mM; L-Arg 50 mM; L-Glu 50 mM; 2-mercaptoethanol 50 mM; DTT 10 mM; EDTA 10 mM

sample_conditions_1: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 288 K

sample_conditions_2: ionic strength: 50 mM; pH: 6.2; pressure: 1 atm; temperature: 303 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_2
3D 1H-15N NOESYsample_1isotropicsample_conditions_2
3D 1H-15N TOCSYsample_1isotropicsample_conditions_2

Software:

SPARKY v2.1, Goddard - data analysis

NMR spectrometers:

  • Bruker DRX 600 MHz
  • Bruker Avance II+ 700 MHz

Related Database Links:

BMRB 16683 16697 17693 16698
PDB
EMBL CAA45680 CAC84353 CAC84354
GB AAA46125 AAA66558
PIR WMBEHA
REF NP_040259
SP P13199