BMRB Entry 15968
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR15968
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Title: Backbone chemical shift assignments for the UBA domain of Swa2p PubMed: 18948116
Deposition date: 2008-09-29 Original release date: 2008-11-05
Authors: Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle
Citation: Matta-Camacho, Edna; Kozlov, Guennadi; Trempe, Jean-Francois; Gehring, Kalle. "Atypical binding of the Swa2p UBA domain to ubiquitin" J. Mol. Biol. 386, 569-577 (2009).
Assembly members:
Swa2p, polymer, 52 residues, Formula weight is not available
Natural source: Common Name: baker Taxonomy ID: 4932 Superkingdom: not available Kingdom: not available Genus/species: Eukaryota Metazoa
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
Swa2p: GPLGSALVDEVKDMEIARLM
SLGLSIEEATEFYENDVTYE
RYLEILKSKQKE
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 93 |
| 15N chemical shifts | 46 |
| 1H chemical shifts | 46 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | polypeptide chain | 1 |
Entities:
Entity 1, polypeptide chain 52 residues - Formula weight is not available
| 1 | GLY | PRO | LEU | GLY | SER | ALA | LEU | VAL | ASP | GLU | ||||
| 2 | VAL | LYS | ASP | MET | GLU | ILE | ALA | ARG | LEU | MET | ||||
| 3 | SER | LEU | GLY | LEU | SER | ILE | GLU | GLU | ALA | THR | ||||
| 4 | GLU | PHE | TYR | GLU | ASN | ASP | VAL | THR | TYR | GLU | ||||
| 5 | ARG | TYR | LEU | GLU | ILE | LEU | LYS | SER | LYS | GLN | ||||
| 6 | LYS | GLU |
Samples:
sample_1: Swa2p, [U-13C; U-15N], 0.5-1.0 mM; HEPES 20 mM; sodium chloride 50 mM
sample_conditions_1: ionic strength: 50 mM; pH: 7; pressure: 1 atm; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
Software:
XEASY, Bartels et al. - data analysis
xwinnmr, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 600 MHz
Related Database Links:
| PDB | |
| DBJ | GAA22540 |
| EMBL | CAY78820 |
| GB | AAB64756 AHY75293 AJP38020 AJU58143 AJU58836 |
| REF | NP_010606 |
| SP | Q06677 |
| TPG | DAA12161 |
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts