BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 30232

Title: ovGRN12-35_3s

Deposition date: 2017-01-17 Original release date: 2018-01-17

Authors: Bansal, P.; Smout, M.; Wilson, D.; Caceres, C.; Dastpeyman, M.; Sotillo, J.; Seifert, J.; Brindley, P.; Loukas, A.; Daly, N.

Citation: Bansal, P.; Smout, M.; Wilson, D.; Caceres, C.; Dastpeyman, M.; Sotillo, J.; Seifert, J.; Brindley, P.; Loukas, A.; Daly, N.. "ovGRN12-35_3s"  . ., .-..

Assembly members:
Granulin, polymer, 24 residues, 2593.018 Da.

Natural source:   Common Name: flatworms   Taxonomy ID: 6198   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Opisthorchis viverrini

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
Granulin: CPDPVYTCRPGQTCCRGLHG YGCC

Data sets:
Data typeCount
13C chemical shifts32
1H chemical shifts127

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11

Entities:

Entity 1, entity_1 24 residues - 2593.018 Da.

1   CYSPROASPPROVALTYRTHRCYSARGPRO
2   GLYGLNTHRCYSCYSARGGLYLEUHISGLY
3   TYRGLYCYSCYS

Samples:

sample_1: D2O 10%; H2O 90%; ov-GRN12-35_3S peptide, NA, 0.5 mM

sample_conditions_1: pH: 5; pressure: 1 atm; temperature: 290 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-13C HSQCsample_1anisotropicsample_conditions_1
2D 1H-1H TOCSYsample_1anisotropicsample_conditions_1
2D 1H-1H NOESYsample_1anisotropicsample_conditions_1
2D 1H-1H COSYsample_1anisotropicsample_conditions_1

Software:

CYANA, Guntert, Mumenthaler and Wuthrich - structure calculation

CcpNMR, CCPN - chemical shift assignment, data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

TOPSPIN v3.5PL6, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 600 MHz