BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4676

Title: Assignment of 1H, 13C and 15N Resonances in Unfolded Apomyoglobin at pH 2.3   PubMed: 11297422

Deposition date: 2000-03-01 Original release date: 2001-08-09

Authors: Yao, Jian; Chung, J.; Eliezer, D.; Wright, Peter; Dyson, Jane

Citation: Yao, Jian; Chung, J.; Eliezer, D.; Wright, Peter; Dyson, H.. "NMR Structural and Dynamic Characterization of the Acid-unfolded State of Apomyoglobin Provides Insights into the Early Events in Protein Folding"  Biochemistry 40, 3561-3571 (2001).

Assembly members:
unfolded myoglobin, polymer, 153 residues, Formula weight is not available

Natural source:   Common Name: sperm whale   Taxonomy ID: 9755   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Physeter catodon

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
unfolded myoglobin: VLSEGEWQLVLHVWAKVEAD VAGHGQDILIRLFKSHPETL EKFDRFKHLKTEAEMKASED LKKHGVTVLTALGAILKKKG HHEAELKPLAQSHATKHKIP IKYLEFISEAIIHVLHSRHP GDFGADAQGAMNKALELFRK DIAAKYKELGYQG

Data sets:
Data typeCount
1H chemical shifts294
13C chemical shifts445
15N chemical shifts147

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1apoMb1

Entities:

Entity 1, apoMb 153 residues - Formula weight is not available

1   VALLEUSERGLUGLYGLUTRPGLNLEUVAL
2   LEUHISVALTRPALALYSVALGLUALAASP
3   VALALAGLYHISGLYGLNASPILELEUILE
4   ARGLEUPHELYSSERHISPROGLUTHRLEU
5   GLULYSPHEASPARGPHELYSHISLEULYS
6   THRGLUALAGLUMETLYSALASERGLUASP
7   LEULYSLYSHISGLYVALTHRVALLEUTHR
8   ALALEUGLYALAILELEULYSLYSLYSGLY
9   HISHISGLUALAGLULEULYSPROLEUALA
10   GLNSERHISALATHRLYSHISLYSILEPRO
11   ILELYSTYRLEUGLUPHEILESERGLUALA
12   ILEILEHISVALLEUHISSERARGHISPRO
13   GLYASPPHEGLYALAASPALAGLNGLYALA
14   METASNLYSALALEUGLULEUPHEARGLYS
15   ASPILEALAALALYSTYRLYSGLULEUGLY
16   TYRGLNGLY

Samples:

sample_1: unfolded myoglobin, [U-95% 13C; U-95% 15N], 0.2 mM

Ex-cond-1: pH: 2.3; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
high field was required for maximum sensitivity for low concentration samples and forsample_1not availableEx-cond-1
maximum proton dispersionsample_1not availableEx-cond-1

Software:

FELIX v97 - transform and plot; data tables assignments were done by hand

NMR spectrometers:

  • Bruker DMX 750 MHz

Related Database Links:

BMRB 1027 1029 1200 1413 1455 1457 1459 1461 1463 1465 1467 1469 1471 15589 16217 16218 16499 16500 16501 1752 2345 2346 2347 2348 2431 2432 2433 2434 291 292 293 40 4061 4062 426 4568 4695
PDB
DBJ BAF03579
GB AAA72199
PRF 742482A
REF NP_001277651
SP P02185

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