BMRB Entry 28033
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR28033
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Backbone assignments for the Asciz QT2-4/LC8 complex PubMed: 32139510
Deposition date: 2019-10-24 Original release date: 2020-03-05
Authors: Reardon, Patrick; Barbar, Elisar; Jara, Kayla; Smith, Delaney
Citation: Reardon, Patrick; Jara, Kayla; Rolland, Amber; Smith, Delaney; Hoang, Hanh; Prell, James; Barbar, Elisar. "The dynein light chain 8 (LC8) binds predominantly "in-register" to a multivalent intrinsically disordered partner" J. Biol. Chem. 295, 4912-4922 (2020).
Assembly members:
QT2-4, polymer, 76 residues, Formula weight is not available
LC8, polymer, 92 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
QT2-4: GAMHMNEVLAPLLRDIETQT
PDTRGDIGTMTDDFPEEQEP
VAVGSHFHAYSETEPMFDLQ
TSAHMYTQTCDDLFEE
LC8: GAHMSDRKAVIKNADMSEEM
QQDAVDCATQALEKYNIEKD
IAAYIKKEFDKKYNPTWHCI
VGRNFGSYVTHETRHFIYFY
LGQVAILLFKSG
- assigned_chemical_shifts
Data type | Count |
13C chemical shifts | 149 |
15N chemical shifts | 53 |
1H chemical shifts | 53 |
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | QT2-4, chain 1 | 1 |
2 | QT2-4, chain 2 | 1 |
3 | LC8, chain 1-1 | 2 |
4 | LC8, chain 1-2 | 2 |
5 | LC8, chain 2-1 | 2 |
6 | LC8, chain 2-2 | 2 |
7 | LC8, chain 3-1 | 2 |
8 | LC8, chain 4-2 | 2 |
Entities:
Entity 1, QT2-4, chain 1 76 residues - Formula weight is not available
1 | GLY | ALA | MET | HIS | MET | ASN | GLU | VAL | LEU | ALA | ||||
2 | PRO | LEU | LEU | ARG | ASP | ILE | GLU | THR | GLN | THR | ||||
3 | PRO | ASP | THR | ARG | GLY | ASP | ILE | GLY | THR | MET | ||||
4 | THR | ASP | ASP | PHE | PRO | GLU | GLU | GLN | GLU | PRO | ||||
5 | VAL | ALA | VAL | GLY | SER | HIS | PHE | HIS | ALA | TYR | ||||
6 | SER | GLU | THR | GLU | PRO | MET | PHE | ASP | LEU | GLN | ||||
7 | THR | SER | ALA | HIS | MET | TYR | THR | GLN | THR | CYS | ||||
8 | ASP | ASP | LEU | PHE | GLU | GLU |
Entity 2, LC8, chain 1-1 92 residues - Formula weight is not available
1 | GLY | ALA | HIS | MET | SER | ASP | ARG | LYS | ALA | VAL | ||||
2 | ILE | LYS | ASN | ALA | ASP | MET | SER | GLU | GLU | MET | ||||
3 | GLN | GLN | ASP | ALA | VAL | ASP | CYS | ALA | THR | GLN | ||||
4 | ALA | LEU | GLU | LYS | TYR | ASN | ILE | GLU | LYS | ASP | ||||
5 | ILE | ALA | ALA | TYR | ILE | LYS | LYS | GLU | PHE | ASP | ||||
6 | LYS | LYS | TYR | ASN | PRO | THR | TRP | HIS | CYS | ILE | ||||
7 | VAL | GLY | ARG | ASN | PHE | GLY | SER | TYR | VAL | THR | ||||
8 | HIS | GLU | THR | ARG | HIS | PHE | ILE | TYR | PHE | TYR | ||||
9 | LEU | GLY | GLN | VAL | ALA | ILE | LEU | LEU | PHE | LYS | ||||
10 | SER | GLY |
Samples:
sample_1: QT2-4, [U-13C; U-15N; U-2H], 0.5 mM; LC8 2.0 mM; beta-mercaptoethanol 10 mM; sodium phosphate 10 mM; sodium chloride 10 mM; sodium azide 1 mM
sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 273 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
3D HNCO | sample_1 | isotropic | sample_conditions_1 |
3D HNCA | sample_1 | isotropic | sample_conditions_1 |
3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
3D HN(CO)CA | sample_1 | isotropic | sample_conditions_1 |
3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
3D HCACO | sample_1 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMRView, Johnson, One Moon Scientific - data analysis
CARA, Keller and Wuthrich - chemical shift assignment
NMR spectrometers:
- Bruker Avance 800 MHz
Download simulated HSQC data in one of the following formats:
CSV: Backbone
or all simulated shifts
SPARKY: Backbone
or all simulated shifts