BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 15183

Title: Backbone and side-chain methyl order parameters and effective correlation times for calmodulin in complex with CaMKKalpha peptide   PubMed: 17637663

Authors: Marlow, Michael; Wand, Joshua

Citation: Frederick, Kendra; Marlow, Michael; Valentine, Kathy; Wand, A. Joshua. "Conformational entropy in molecular recognition by proteins"  Nature 448, 325-329 (2007).

Assembly members:
CaMKKalpha, polymer, 28 residues, Formula weight is not available
calmodulin, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):
CaMKKalpha: GSVKLIPSWTTVILVKSMLR KRSFGNPF
calmodulin: ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data sets:
Data typeCount
order parameters205

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1calmodulin2
2CaMKKalpha1

Entities:

Entity 2, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Entity 1, CaMKKalpha 28 residues - Formula weight is not available

1   GLYSERVALLYSLEUILEPROSERTRPTHR
2   THRVALILELEUVALLYSSERMETLEUARG
3   LYSARGSERPHEGLYASNPROPHE

Samples:

sample_1: calmodulin, [U-100% 15N], 1.0 mM; CaMKKalpha1.1 – 1.2 mM

sample_2: calmodulin, [U-100% 13C; U-100% 15N; 60% 2H], 1.0 mM; CaMKKalpha1.1 – 1.2 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1
2D 1H-13C HSQCsample_2isotropicsample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRDraw, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - peak picking

SPARKY, Goddard - peak picking

NMR spectrometers:

  • Varian INOVA 500 MHz
  • Varian INOVA 750 MHz

Related Database Links:

GB AAC31608 AAB46910 AAC42070 EDM05132 EGW04580 EHB05791 AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PDB
DBJ BAA75246 BAA08302 BAA11896 BAB23462 BAB28116 BAB28319
PRF 2120334A
REF NP_113850 XP_002718907 XP_003416891 XP_003469697 XP_003509228 NP_001008160 NP_001009759 NP_001039714 NP_001080864 NP_001084025
SP P97756 P02594 P21251 P62144 P62149 P62151
BMRB 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 16418 16465 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 6541 6798 6802 6825 6830 6831 7018 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425
EMBL CAA32050 CAA32062 CAA32119 CAA32120 CAA32478
PIR JC1305 MCON
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988