BMRB Entry 4270
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4270
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Title: Chemical Shift Assignment of Ca2+/calmodulin (1H, 13C, and 15N) Complexed with Its Binding Domain from Rat Ca2+/calmodulin Dependent Protein Kinase Kinase (1H).
Deposition date: 1998-11-18 Original release date: 2007-07-13
Authors: Osawa, Masanori; Tokumitsu, Hiroshi; Swindells, Mark; Kurihara, Hiroyuki; Orita, Masaya; Shibanuma, Tadao; Furuya, Toshio; Ikura, Mitsuhiko
Citation: Osawa, Masanori; Tokumitsu, Hiroshi; Swindells, Mark; Kurihara, Hiroyuki; Orita, Masaya; Shibanuma, Tadao; Furuya, Toshio; Ikura, Mitsuhiko. "A Novel Target Recognition Revealed by Calmodulin in Complex with Ca2+-calmodulin-dependent Kinase Kinase" Nat. Struct. Biol. 6, 819-824 (1999).
Assembly members:
calmodulin, polymer, 148 residues, 40.078 Da.
Ca2+/calmodulin dependent protein kinase kinase complex, polymer, 26 residues, 40.078 Da.
CALCIUM ION, non-polymer, 40.078 Da.
Natural source: Common Name: African clawed frog Taxonomy ID: 8355 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Xenopus laevis
Experimental source: Production method: recombinant technology Host organism: Escherichia coli
Entity Sequences (FASTA):
calmodulin: ADQLTEEQIAEFKEAFSLFD
KDGDGTITTKELGTVMRSLG
QNPTEAELQDMINEVDADGN
GTIDFPEFLTMMARKMKDTD
SEEEIREAFRVFDKDGNGYI
SAAELRHVMTNLGEKLTDEE
VDEMIREADIDGDGQVNYEE
FVQMMTAK
Ca2+/calmodulin dependent protein kinase kinase complex: VKLIPSWTTVILVKSMLRKR
SFGNPF
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 601 |
| 15N chemical shifts | 153 |
| 1H chemical shifts | 1137 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | calmodulin | 1 |
| 2 | camkk | 2 |
Entities:
Entity 1, calmodulin 148 residues - 40.078 Da.
| 1 | ALA | ASP | GLN | LEU | THR | GLU | GLU | GLN | ILE | ALA | ||||
| 2 | GLU | PHE | LYS | GLU | ALA | PHE | SER | LEU | PHE | ASP | ||||
| 3 | LYS | ASP | GLY | ASP | GLY | THR | ILE | THR | THR | LYS | ||||
| 4 | GLU | LEU | GLY | THR | VAL | MET | ARG | SER | LEU | GLY | ||||
| 5 | GLN | ASN | PRO | THR | GLU | ALA | GLU | LEU | GLN | ASP | ||||
| 6 | MET | ILE | ASN | GLU | VAL | ASP | ALA | ASP | GLY | ASN | ||||
| 7 | GLY | THR | ILE | ASP | PHE | PRO | GLU | PHE | LEU | THR | ||||
| 8 | MET | MET | ALA | ARG | LYS | MET | LYS | ASP | THR | ASP | ||||
| 9 | SER | GLU | GLU | GLU | ILE | ARG | GLU | ALA | PHE | ARG | ||||
| 10 | VAL | PHE | ASP | LYS | ASP | GLY | ASN | GLY | TYR | ILE | ||||
| 11 | SER | ALA | ALA | GLU | LEU | ARG | HIS | VAL | MET | THR | ||||
| 12 | ASN | LEU | GLY | GLU | LYS | LEU | THR | ASP | GLU | GLU | ||||
| 13 | VAL | ASP | GLU | MET | ILE | ARG | GLU | ALA | ASP | ILE | ||||
| 14 | ASP | GLY | ASP | GLY | GLN | VAL | ASN | TYR | GLU | GLU | ||||
| 15 | PHE | VAL | GLN | MET | MET | THR | ALA | LYS |
Entity 2, camkk 26 residues - 40.078 Da.
| 1 | VAL | LYS | LEU | ILE | PRO | SER | TRP | THR | THR | VAL | ||||
| 2 | ILE | LEU | VAL | LYS | SER | MET | LEU | ARG | LYS | ARG | ||||
| 3 | SER | PHE | GLY | ASN | PRO | PHE |
Samples:
sample_one: calmodulin, [U-13C, U-15N], 1.5 mM; Ca2+/calmodulin dependent protein kinase kinase complex 1.875 mM; potassium chloride 100 mM; calcium chloride 10.6 mM
sample_conditions_one: pH: 6.7; temperature: 303 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| not available | sample_one | not available | sample_conditions_one |
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 25253 25257 26503 26626 26627 4056 4284 4310 |
| PDB | |
| DBJ | BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 |
| EMBL | CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 |
| GB | AAA35635 AAA35641 AAA37365 AAA40862 AAA40863 |
| PIR | JC1305 MCON |
| PRF | 0409298A 0608335A |
| REF | NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 |
| SP | O02367 O16305 O96081 P02594 P05932 |
| TPG | DAA13808 DAA18029 DAA19590 DAA24777 DAA24988 |
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