BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 5227

Title: Dissection of the Pathway of Molecular Recognition by Calmodulin   PubMed: 11851407

Authors: Kranz, James; Flynn, Peter; Fuentes, Ernesto; Wand, A.

Citation: Kranz, James; Flynn, Peter; Fuentes, Ernesto; Wand, A.. "Dissection of the Pathway of Molecular Recognition by Calmodulin"  Biochemistry 41, 2599-2608 (2002).

Assembly members:
Calmodulin recognition element from smooth muscle myosin light chain kinase, polymer, 21 residues, 2336 Da.
calmodulin (chicken), polymer, 148 residues, 16700 Da.

Natural source:   Common Name: Chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):
Calmodulin recognition element from smooth muscle myosin light chain kinase: GSARRKWQKTGHAVRAIGRL S
calmodulin (chicken): ADQLTEEQIAEFKEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGYI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data sets:
Data typeCount
1H chemical shifts135
13C chemical shifts87
15N chemical shifts25

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1smMLCKp peptide1
2calmodulin2

Entities:

Entity 1, smMLCKp peptide 21 residues - 2336 Da.

1   GLYSERALAARGARGLYSTRPGLNLYSTHR
2   GLYHISALAVALARGALAILEGLYARGLEU
3   SER

Entity 2, calmodulin 148 residues - 16700 Da.

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHELYSGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYTYRILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Samples:

sample_1: Calmodulin recognition element from smooth muscle myosin light chain kinase, [U-99% 13C; U-99% 15N], 2.00 mM; calmodulin (chicken) 2.25 mM; CaCl2 20 mM; KCl 100 mM; Bis-tris 20 mM; NaN3 0.02%; D2O 7%

Ex-cond_1: pH*: 6.0 na; temperature: 303 K; ionic strength: 0.16 M

Experiments:

NameSampleSample stateSample conditions
1H-15N HSQCnot availablenot availablenot available
1H-13C CT-HSQCnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCOnot availablenot availablenot available
HN(CA)COnot availablenot availablenot available
HBHACONHnot availablenot availablenot available
HCCHTOCSYnot availablenot availablenot available
CH3-filtered (1H13C13C) HCCHTOCSYnot availablenot availablenot available
CCC TOCSY NNHnot availablenot availablenot available

Software:

FELIX v98.0 - processing, peak picking, assignments

NMR spectrometers:

  • Varian INOVA 750 MHz

Related Database Links:

BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 547 6541 6778 6798 6802 6825 6830 6831 7018 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425
PDB
DBJ BAA08302 BAA11896 BAA19786 BAA19787 BAA19788 BAB28116 BAB28319 BAA11896 BAB23462 BAA08302
EMBL CAA10601 CAA32050 CAA32062 CAA32119 CAA32120 CAA32478 CAA32119 CAA32120 CAA32050 CAA32062
GB AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PIR JC1305 MCON JC1305 MCON
PRF 0409298A 0608335A
REF NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234 NP_001084025 NP_001039714 NP_001080864 NP_001008160 NP_001009759
SP O02367 O16305 O96081 P02594 P05932
TPG DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
SWISS-PROT P62149 P62151 P21251 P62144 P02594
GenBank AAA40862 AAA40863 AAA35641 AAA37365 AAA35635
100.00 148
. .
-[1-R- (3,4-Bis-Butoxyphenyl)-Ethyl]-Propylenediamine
1NWD .
1PRW .
1LVC .
1MUX .
1L7Z .
1LIN .
1IWQ .
1K90 .
1G4Y .
1IQ5 .
1CTR .
1DMO .
1CM1 .
1CM4 .
1CKK .
1CLL .
1CFD .
1CFF .
1A29 .
1CFC .
6023 .
6541 .
5480 .
5770 .
5286 .
5287 .
4310 .
4970 .
4270 .
4284 .
15191 .
4056 .
15187 .
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15183 .
15184 .