BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 6825

Title: 1H Chemical Shifts of CaD Peptide in CaM/Peptide Mixture (1/30) at pH 6.5, 288 K.   PubMed: 8110748

Authors: Vogel, Hans; Zhang, Mingjie

Citation: Vogel, Hans; Zhang, Mingjie. "The Calmodulin-Binding Domain of Caldesmon Binds to Calmodulin in an alpha-Helical Conformation"  Biochemistry 33, 1163-1171 (1994).

Assembly members:
CaD peptide, polymer, 17 residues, Formula weight is not available
calmodulin, polymer, 148 residues, Formula weight is not available

Natural source:   Common Name: not available   Taxonomy ID: not available   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
CaD peptide: GVRNIKSMWEKGNVFSS
calmodulin: ADQLTEEQIAEFYEAFSLFD KDGDGTITTKELGTVMRSLG QNPTEAELQDMINEVDADGN GTIDFPEFLTMMARKMKDTD SEEEIREAFRVFDKDGNGFI SAAELRHVMTNLGEKLTDEE VDEMIREADIDGDGQVNYEE FVQMMTAK

Data sets:
Data typeCount
1H chemical shifts101

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1caldesmon peptide1
2calmodulin2

Entities:

Entity 1, caldesmon peptide 17 residues - Formula weight is not available

1   GLYVALARGASNILELYSSERMETTRPGLU
2   LYSGLYASNVALPHESERSER

Entity 2, calmodulin 148 residues - Formula weight is not available

1   ALAASPGLNLEUTHRGLUGLUGLNILEALA
2   GLUPHETYRGLUALAPHESERLEUPHEASP
3   LYSASPGLYASPGLYTHRILETHRTHRLYS
4   GLULEUGLYTHRVALMETARGSERLEUGLY
5   GLNASNPROTHRGLUALAGLULEUGLNASP
6   METILEASNGLUVALASPALAASPGLYASN
7   GLYTHRILEASPPHEPROGLUPHELEUTHR
8   METMETALAARGLYSMETLYSASPTHRASP
9   SERGLUGLUGLUILEARGGLUALAPHEARG
10   VALPHEASPLYSASPGLYASNGLYPHEILE
11   SERALAALAGLULEUARGHISVALMETTHR
12   ASNLEUGLYGLULYSLEUTHRASPGLUGLU
13   VALASPGLUMETILEARGGLUALAASPILE
14   ASPGLYASPGLYGLNVALASNTYRGLUGLU
15   PHEVALGLNMETMETTHRALALYS

Samples:

sample_1: CaM-CaD peptide 1.4 mM; CaM mM; KCl 0.1 M

conditions_1: pH: 6.5; temperature: 288 K

Experiments:

NameSampleSample stateSample conditions
unknownsample_1isotropicconditions_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz

Related Database Links:

DBJ BAA04538 BAA04539 BAA04540 BAA14418 BAA14419 BAA08302 BAA11896 BAA19786 BAA19787 BAA19788
EMBL CAD98104 CAH92562 CAI46261 CAA10601 CAA32050 CAA32062 CAA32119 CAA32120
GB AAA35636 AAA48649 AAA48810 AAA48811 AAA48936 AAA35635 AAA35641 AAA37365 AAA40862 AAA40863
PRF 1803426A
REF NP_001126502 NP_001233244 NP_001270636 NP_004333 NP_149129 NP_001008160 NP_001009759 NP_001027633 NP_001039714 NP_001040234
SP P12957 P13505 Q05682 O02367 O16305 O96081 P02594 P05932
TPG DAA30350 DAA13808 DAA18029 DAA19590 DAA24777 DAA24988
BMRB 15183 15184 15185 15186 15187 15188 15191 15470 15624 15650 15852 1634 16418 16465 1648 16764 17264 17360 17771 17807 18027 18028 18556 19036 19238 19586 19604 26503 4056 5227 547 6541 6778 6798 6802 6830 6831 7018 7028 7029 7030 7031 7416 7417 7418 7423 7424 7425
PDB
PIR JC1305 MCON