BMRB Entry 17360

Assembly:

Entities:

Entity 1, entity_1 148 residues - 16721.465 Da.

Entity 2, entity_2 22 residues - 2606.146 Da.

N-terminal acetylation C-terminal amidation

Entity 3, CALCIUM ION_1 - Ca - 40.078 Da.

Samples:

sample_1: entity_1, [U-13C; U-15N], 0.95 mM; entity_2 1.05 mM; CALCIUM ION 4 mM; DSS 0.5 mM; potassium chloride 100 mM; Bis-Tris 20 mM; sodium azide 0.03%

sample_2: entity_1, [U-2H; U-15N], 0.66 mM; entity_2 0.40 mM; Bis-Tris 20 mM; potassium chloride 100 mM; CALCIUM ION 4 mM; sodium azide 0.03%; DSS 0.5 mM

sample_3: entity_1, [1H/13C-methyl Met; U-2H; U-15N], 0.64 mM; entity_2 0.83 mM; Bis-Tris 20 mM; potassium chloride 100 mM; CALCIUM ION 4 mM; sodium azide 0.03%; DSS 0.5 mM

sample_4: entity_1, [U-13C; U-15N], 0.95 mM; entity_2 1.05 mM; Bis-Tris 20 mM; potassium chloride 300 mM; CALCIUM ION 4 mM; sodium azide 0.03%; DSS 0.5 mM

sample_5: entity_1, [U-13C; U-15N], 0.95 mM; entity_2 1.05 mM; Bis-Tris 20 mM; potassium chloride 300 mM; CALCIUM ION 4 mM; sodium azide 0.03%; DSS 0.5 mM; Pf1 phage 16 w/v

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

sample_conditions_2: ionic strength: 0.3 M; pH: 6.8; pressure: 1 atm; temperature: 303 K

Experiments:

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking

CYANA, Guntert, Mumenthaler and Wuthrich - chemical shift assignment, refinement, structure solution

X-PLOR NIH, Schwieters, Kuszewski, Tjandra and Clore - refinement, structure solution

ProcheckNMR, Laskowski and MacArthur - data analysis

TALOS, Cornilescu, Delaglio and Bax - data analysis

Molmol, Koradi, Billeter and Wuthrich - data analysis

xwinnmr, Bruker Biospin - collection

NMR spectrometers:

• Bruker Avance 500 MHz