BMRB Entry 16755
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR16755
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
NMR-STAR v2.1 text file (deprecated)
XML gzip file.
RDF gzip file.
All files associated with the entry
Title: Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution PubMed: 7789539
Deposition date: 2010-03-03 Original release date: 2010-03-24
Authors: Kobayashi, Naohiro; Honda, Shinya; Yoshii, Hirofumi; Uedaira, Hatsuho; Munekata, Eisuke
Citation: Kobayashi, Naohiro; Honda, Shinya; Yoshii, Hirofumi; Uedaira, Hatsuho; Munekata, Eisuke. "Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution" FEBS Lett. 366, 99-103 (1995).
Assembly members:
N40, polymer, 40 residues, Formula weight is not available
C16, polymer, 16 residues, Formula weight is not available
Natural source: Common Name: Streptococcus Taxonomy ID: 1301 Superkingdom: Bacteria Kingdom: not available Genus/species: Streptococcus not available
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
N40: DTYKLILNGKTLKGETTTEA
VDAATAEKVFKQYANDNGVD
C16: GEWTYDDATKTFTVTE
Additional metadata:
Assembly:
Entity Assembly ID | Entity Name | Entity ID |
---|---|---|
1 | N40 | 1 |
2 | C16 | 2 |
Entities:
Entity 1, N40 40 residues - Formula weight is not available
1 | ASP | THR | TYR | LYS | LEU | ILE | LEU | ASN | GLY | LYS | |
2 | THR | LEU | LYS | GLY | GLU | THR | THR | THR | GLU | ALA | |
3 | VAL | ASP | ALA | ALA | THR | ALA | GLU | LYS | VAL | PHE | |
4 | LYS | GLN | TYR | ALA | ASN | ASP | ASN | GLY | VAL | ASP |
Entity 2, C16 16 residues - Formula weight is not available
1 | GLY | GLU | TRP | THR | TYR | ASP | ASP | ALA | THR | LYS | ||||
2 | THR | PHE | THR | VAL | THR | GLU |
Samples:
sample_1: N40 1.8 mM; C16 1.8 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%
sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K
Experiments:
Name | Sample | Sample state | Sample conditions |
---|---|---|---|
2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
2D HOHAHA | sample_1 | isotropic | sample_conditions_1 |
2D ROESY | sample_1 | isotropic | sample_conditions_1 |
2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
Software:
MOLSCRIPT, Kraulis, 1991 - visualization
NMR spectrometers:
- Bruker AM 500 MHz
- Bruker AMX 500 MHz
- Bruker AMX 600 MHz