BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
Member of WWPDB

BMRB Entry 16755

Title: Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution   PubMed: 7789539

Authors: Kobayashi, Naohiro; Honda, Shinya; Yoshii, Hirofumi; Uedaira, Hatsuho; Munekata, Eisuke

Citation: Kobayashi, Naohiro; Honda, Shinya; Yoshii, Hirofumi; Uedaira, Hatsuho; Munekata, Eisuke. "Complement assembly of two fragments of the streptococcal protein G B1 domain in aqueous solution"  FEBS Lett. 366, 99-103 (1995).

Assembly members:
N40, polymer, 40 residues, Formula weight is not available
C16, polymer, 16 residues, Formula weight is not available

Natural source:   Common Name: Streptococcus   Taxonomy ID: 1301   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Streptococcus not available

Experimental source:   Production method: chemical synthesis

Entity Sequences (FASTA):
N40: DTYKLILNGKTLKGETTTEA VDAATAEKVFKQYANDNGVD
C16: GEWTYDDATKTFTVTE

Data sets:
  • assigned_chemical_shifts
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N401
2C162

Entities:

Entity 1, N40 40 residues - Formula weight is not available

1   ASPTHRTYRLYSLEUILELEUASNGLYLYS
2   THRLEULYSGLYGLUTHRTHRTHRGLUALA
3   VALASPALAALATHRALAGLULYSVALPHE
4   LYSGLNTYRALAASNASPASNGLYVALASP

Entity 2, C16 16 residues - Formula weight is not available

1   GLYGLUTRPTHRTYRASPASPALATHRLYS
2   THRPHETHRVALTHRGLU

Samples:

sample_1: N40 1.8 mM; C16 1.8 mM; phosphate buffer 50 mM; H2O 90%; D2O 10%

sample_conditions_1: pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D DQF-COSYsample_1isotropicsample_conditions_1
2D HOHAHAsample_1isotropicsample_conditions_1
2D ROESYsample_1isotropicsample_conditions_1
2D 1H-1H NOESYsample_1isotropicsample_conditions_1

Software:

MOLSCRIPT, Kraulis, 1991 - visualization

NMR spectrometers:

  • Bruker AM 500 MHz
  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz

Related Database Links:

BMRB 15156 15380 16053 16444 16627 16873 16882 16958 17810 18397 19394 26630 15156 15380 16053 1639 16444 16627 16670 16873 16882 16958 17810 18397 19394 5151 5569 5654 5875 6280 6988 7280
PDB
GB AAA26599 AAA26600 AAY41168 AAA03280 AHW57457
EMBL CAA37410
PIR A45063